When the RPA-LFD was applied in parallel with serological ELISA for the detection of CAEV in field samples, the RPA-LFD assay exhibited a higher sensitivity than the traditional method, and 82% of the 200 samples collected in Taiwan were found to be positive. Given its simplicity and portability, this RPA-LFD protocol can serve as an alternative tool to ELISA for the primary screening of CAEV, one that is suitable for both laboratory and field application. Furthermore, there was no cross-reaction with other tested viruses, including goat pox virus and bovine leukemia virus. Under the optimal incubation conditions, specifically, 30min at 37Â☌ for RPA followed by 5min at room temperature for LFD, the assay was found to be sensitive to a lower limit of 80pg of total DNA and 10 copies of plasmid DNA. Hence, a more efficient detection platform based on recombinase polymerase amplification (RPA) and a lateral flow dipstick (LFD) was developed in the present study for detecting the proviral DNA of caprine arthritis-encephalitis virus (CAEV). The prognosis for the encephalitic form is normally poor, and this form of the disease has caused substantial economic losses for goat farmers. This persistent viral infection results in arthritis in adult goats and encephalitis in lambs. Tu, Po-An Shiu, Jia-Shian Lee, Shu-Hwae Pang, Victor Fei Wang, De-Chi Wang, Pei-HwaĬaprine arthritis-encephalitis (CAE) in goats is a complex disease syndrome caused by a lentivirus. PMID:1335836ĭevelopment of a recombinase polymerase amplification lateral flow dipstick (RPA-LFD) for the field diagnosis of caprine arthritis-encephalitis virus (CAEV) infection. However, the results did indicate that all isolates were not the same, except for two from the same laboratory where the source of pestivirus contamination may have been common. The panel of monoclonal antibodies could not type the isolates as being more related to bovine virus diarrhea virus or border disease virus. All of the viral stocks examined were found to contain noncytopathic pestivirus contaminants. Pestivirus is a common contaminant in maedi-visna and caprine arthritis-encephalitis virus stocks.Įight different laboratory stocks of maedi-visna or caprine arthritis-encephalitis virus were examined for the presence of pestiviruses by a fixed-cell immunoperoxidase assay with polyclonal and monoclonal antibodies. 1Department of Animal Production and Health, Animal Parasitology and Microbiology Research Unit, Federal University of Technology, P M B 704, . Affinity (tropism) of caprine arthritis encephalitis virus for brain cells. The specific brain cell types infected by the (CAE) virus were determined using reverse-transcription polymerase chain reaction (RTPCR) and transmission .Īffinity (tropism) of caprine arthritis encephalitis virus for brain cells Caprine Arthritis Encephalitis (CAE) virus- a retrovirus affecting goats. In this study, explant cultures prepared from the brain of new-born goat-kid were infected with.
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